Generator

Part:BBa_I766258:Design

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-25)


OspF-EE under inducible promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1540
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1540
    Illegal NotI site found at 2099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2082
    Illegal XhoI site found at 1180
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1540
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1540
    Illegal NgoMIV site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

see wiki for info on combinatorial cloning techniques


Source

composite

References